6X His tag Mouse monoclonal Antibody
Fig1: Western blot analysis on recombinant protein with His tag using anti-6
Host Species; Species ReactivityMouse
Immunogensynthetic Peptide (KLH-coupled)
Purification; FormulationProG affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
BackgroundA variety of plasmids contain DNA that encodes a tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. This antibody can be used to detect the carboxyl-terminal 6×his tag in recombinant protein.(M0812-3)