β-tubulin Mouse monoclonal Antibody IgG1

SKU: BA110005-100µl
$279.00Price

Fig1: Western blot analysis of β-tubulin on different cell lysates using anti-β-tubulin antibody at 1/5000 dilution.

Positive control: 

  Lane 1: NCCIT 

  Lane 2: NIH/3T3 

  Lane 3: PC12 

  Lane 4: Mouse heart 

  Lane 5: F9 

  Lane 6: zebrafish brain 

  Lane 7: Hela

Fig2: ICC staining β-tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC stainingβ-tubulin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA110005
Size
  • Host Species; Species Reactivity

    Mouse; Human, Mouse, Rat, Zebrafish
  • Immunogen

    Peptide
  • Application Summary

    WB, ICC, IHC, FC
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Tubulin beta chain,Tubulin beta-5 chain
  • Background

    Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.(EM0103)

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