Custom Protein Production
Bon Opus Biosciences is a specialty company for recombinant protein and antibody production services. Our protein team is led by a group of scientists with years of experience in recombinant expression and relevant process development. The team has successfully produced thousands of proteins in the past, and they are more than happy to take on a variety of recombinant expression projects, ranging from trial expression of rare protein variants, to gram-level production of antibodies and enzymes. The success rate of our team of protein purification and expression specialists is greater than 90%.
Features of our full-scale custom protein services include:
Low endotoxin production (to 1EU/mg)
Purification process: affinity purification, gel filtration, IEX
Scale up production (up to gram level)
Refolding (for E. coli expression)
Biotinylation service available (site-specific and NHS-based)
Bundle with gene synthesis and cloning work
At Bon Opus, we ensure a simple and transparent process. In order to accomplish this, we designate one Ph.D.-level project manager to work with the customer throughout the entirety of the project.
The normal workflow is as follows: our team receives the initial inquiry and assigns a project manager, who will contact the customer within 24 hours. The project manager will propose a work plan and final quote; in turn, the customer will review the plan and submit the PO. Production commences immediately, and both the product and product report are delivered in the discussed timeframe.
Based on the particular nature of the project, you may choose to use one or more of the following expression platforms:
For each of these systems, we have developed in-house processes and reagents - including a variety of vectors, signal peptides, bacterial strains, and unique designs - in order to achieve optimized production efficiency.
Our current $1,500 production package features two expression system options: 2 mg of protein in an E. coli system or 200 ug in a mammalian system. Turnaround time for both options is 6-8 weeks.
WHAT PEOPLE SAY
Hadassah Medical Center for Cancer Immunotherapy
We have a long and fruitful working relationship with the Bon Opus team. We have a tremendous appreciation for their valuable scientific support and excellent recombinant protein production. We received several batches of our custom-made protein with very good reproducibility. We also want to compliment the team's technical and scientific support that was always available. We will definitely continue working with Bon Opus in the future.
Dr. Luis Gimenez-Lirola,
Iowa State University
My long-term relationship with Bon Opus Biosciences is based on high quality work, flexibility, quick turnaround times, and outstanding personal customer service. Aside from excelling in customized projects, they offer a broad portfolio of products at extremely competitive prices. They are always available for questions and open to discuss alternatives for custom projects that are technically complicated. I will definitively continue working with them as they have become a crucial part of my research workflow.
Aleta Biotherapeutics has a very good working relationship with Bon Opus. We have obtained more than 5 batches of protein produced by Bon Opus, including His tagged recombinant proteins and antibody-based proteins ranging from a 5 mg to 50 mg scale. The proteins produced by Bon Opus were of high quality with very low percentage aggregation and low endotoxin level. The representative we worked with at Bon Opus was very receptive to what we required for the product and delivered it to our satisfaction. We are happy to have Bon Opus as a reliable partner and will continue the collaboration with Bon Opus for future projects.
Dr. Harbani Malik,
Bon Opus Biosciences has been able to provide us several reagents for our research projects. They are easy to work with and provide excellent communication updates throughout the process each time. When technical difficulties/problems arose during the project, the team was able to quickly provide several great suggestions for different strategies regarding protein production. [Bon Opus] also has highly competitive pricing.