The Journal of Immunology - August 1, 2020
A New Tool to Understand IL-17F
"The ST2 stromal cell line was cultured in a-MEM (Sigma-Aldrich,
St. Louis, MO) with L-glutamine, antibiotics, and 10% FBS. A total of
5 3 104 cells were seeded into six-well plates prior to cytokine stimulation.
rIL-17F.S65L and IL-17F were synthesized by Bon Opus Biosciences
(Millburn, NJ) ..."
Journal of Proteomics, 215 - March 20 2020
"The rabbit alpha-pT280TLT-1 antibody (used at 1:500) was raised against the sequence CSKPVpTYATVIFPGGNK (human TLT-1 peptide) by Bon Opus Biosciences (Millburn, NJ, USA)."
33, 1878-1887 - July 04 2019
"Proteins were transferred onto nitrocellulose membranes that had been saturated by incubation with 10% non‐fat dry milk in 1× PBS overnight at 4°C
with the following antibodies... rabbit polyclonal anti‐NF‐κB p65 (1:6000 v/v, Bon Opus Biosciences LLC, Summit, New Jersey, USA)... protein
expression was performed on total protein fractions of homogenates."
Nature Genetics - Articles
May 20, 2019
Genome-scale screens identify JNK–JUN signaling
as a barrier for pluripotency exit and endoderm
"For the H1 line, we typically plated 0.1 million cells to accommodate the
higher proliferation rate compared with other lines. For differentiation in 150-mm
dishes, we multiply the numbers by a factor of 15 based on the numbers from sixwell
plates. Twenty-four hours later, cells were washed with PBS once and culture
medium was changed to Advanced RPMI (12633012; Thermo Fisher Scientific)
with penicillin-streptomycin (15070063; Thermo Fisher Scientific), GlutaMAX
(35050079; Thermo Fisher Scientific), 0.003% BSA 15260037; Thermo Fisher
Scientific), 5 μM CHIR99021 (4423; Tocris) and 100 (high) or 20 (low) ng ml−1
Activin A (12014E (PeproTech); BP08001 (Bon Opus Biosciences))."
Recombinant Human Activin A
Journal of Pharmaceutical and Biomedical Analysis - November 21 2019
"Despite the comparable migration behaviors of native proteins on the two platforms, the correlation has not been evaluated systematically for biotherapeutic proteins, which are often confounded by the presence of glycans and disulfides. Here we studied various mammalian glycoproteins using the two techniques under both reduced and non-reduced conditions... Human erythropoietin (EPO), CTLA4-Ig were from Bon Opus Biosciences (Millburn, NJ)."
Recombinant Human Erythropoietin/EPO (C-6His)
Recombinant Human Cytotoxic T-Lymphocyte Protein 4/CTLA-4/CD152 (C-Fc)
Nature International Journal of Medicine, 549, 523-527 - September 28 2017
"Supernatants were collected and assayed for mouse TNF-α , IL-1α , and IL-1β using single cytokine ELISAs (Bon Opus Biosciences) according to the manufacturer’s instructions. All cytokine levels were normalized to microglial protein levels determined by BCA assay."
Mouse Tumor Necrosis Factor-alpha (TNF-α) ELISA Kit
Mouse Interleukin-1α (IL-1α) ELISA Kit
Mouse Interleukin-1β (IL-1β) ELISA Kit