c-RAF, Raf-1, RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, RAF1
Ship on Dry Ice
LinkLight™ U2-OS RAF1 / 14-3-3 Human Kinase Cell Line is a stable monoclonal cell line in U2-OS cells, targeting Human RAF1 pathway via 14-3-3. The proprietary LinkLight™ technology uses a standard Luciferase Assay as the detection method.
RAF1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. ERKs can be pleiotropic effectors of cell physiology once activated, and can control gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Host Cell Lines:
Standard Luciferase Assay System for mammalian cells should be followed according to Assay kit manufacturer protocol.