Ship on Dry Ice
LinkLight™ U2-OS HTR2C / β-arrestin Cell Line is a stable monoclonal cell line in U2-OS cells, targeting Human HTR2C pathway via b-arrestin. The proprietary LinkLight™ technology uses a standard Luciferase Assay as the detection method.
5HT2C (HTR2C) is a G-protein coupled receptor for 5-hydroxytryptamine (serotonin). It also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase (PI3K) and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone. Plays a role in the regulation of appetite and eating behavior, responses to anxiogenic stimuli and stress. Plays a role in insulin sensitivity and glucose homeostasis.
Host Cell Lines:
Standard Luciferase Assay System for mammalian cells should be followed according to Assay kit manufacturer protocol.