MAP3K5, MEKK5, Mitogen-activated protein kinase kinase 5, apoptosis signal-regulating kinase 1, ASK1, ASK 1
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LinkLight™ U2-OS ASK1 / 14-3-3 Human Kinase Cell Line is a stable monoclonal cell line in U2-OS cells, targeting Human ASK1 pathway via b-arrestin. The proprietary LinkLight™ technology uses a standard Luciferase Assay as the detection method.
ASK1, also known as MAP3K5, contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Host Cell Lines:
Standard Luciferase Assay System for mammalian cells should be followed according to Assay kit manufacturer protocol.